flag-HRP Mouse Monoclonal Antibody
Catalog NO.：BE7003
Applications ：WB
Reactivity ：ALL
| 貨號 | 規格 | 品牌 | 庫存 | 價格 | 數量 | 操作 |
|---|---|---|---|---|---|---|
| BE7003-100 | 100ul/支 | EASYBIO |
|
The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.
| Product Name: | flag-HRP Mouse Monoclonal Antibody |
|---|---|
| Isotype: | IgG1 |
| Storage Buffer : | PBS, pH 7.4, containing 0.02% kathon as Preservative and 50% Glycerol |
| Storage instructions: | -20°C. Do not aliquot the antibody |
| Recommended dilutions: | WB: 1:2,000-5,000 |
| Optimal dilutions should be determined by the end user. | |
| Specificity： | The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins. |
| Alternative Names： | DDDDK epitope tag, DYKDDDDK epitope tag, ECS epitope tag |
| Form: | Liquid |
| Reactivity: | ALL |
[2]Huang, J., Zhao, Y., Liu, S., Chen, Y., Du, M., Wang, Q., Zhang, J., Yang, X., Chen, J., & Zhang, X. (2024). RH20, a phase-separated RNA helicase protein, facilitates plant resistance to viruses. Plant science : an international journal of experimental plant biology, 347, 112176. https://doi.org/10.1016/j.plantsci.2024.112176
[3]Huang, J., Du, J., Liu, Y., Lu, L., Xu, Y., Shi, J., Liu, Q., Li, Q., Liu, Y., Chen, Y., Du, M., Zhao, Y., Huo, L., Wang, W., Ding, C., Wei, L., Wu, J., Yuan, Y. W., Chen, J., Li, R., … Zhang, X. (2025). RH3 enhances antiviral defense by facilitating small RNA loading into Argonaute 2 at endoplasmic reticulum-chloroplast membrane contact sites. Nature communications, 16(1), 1953. https://doi.org/10.1038/s41467-025-57296-6
